Understanding morphological evolution in lichens

2012 REU Project:

Lichen-forming fungi are unique among the fungal kingdom since these organisms form specific vegetative thalli to host their photosynthetic symbiotic partner (wither algae or cyanobacteria) that provide energy for the symbiotic system. These thalli have certain morphologies and include adaptations, such as a cortex covering the algal layer to protect them from UV radiations. Other adaptations include crystals in the algal layer to increase the light intensity for species growing in shady habitats in tropical rainforests. Currently, our knowledge about the evolution of these adaptations is very poor. In a project focusing on the tropical lichen family Graphidaceae, this project will address the origin of morphological adaptations in a phylogenetic context. DNA sequence data will be used to address these issues. The lichens selected for this group belong to the so-called Topeliopsis clade within the family Graphidaceae (Ostropales).

Research methods and techniques: REU participants in this project will receive training in molecular and organismal research methods. They will learn how important a combination of both methods is for an understanding of the evolution of the diversity of life. The training will include introduction to the literature and handling of herbarium specimens. Chemical examination will include chromatographic methods, such as HPTLC and HPLC. Molecular methods will include DNA isolation, PCR and subsequent direct sequencing of certain gene regions. Subsequently, the analysis of DNA sequence data will be performed.

Curator/Advisor: Dr. Thorsten Lumbsch, Botany